International post-graduate course on Protein Quality Control
Scientists currently using or intending to use purified proteins in their laboratory experiments are welcome to participate to this course.
By the end of the course, participants should be familiar with a variety of techniques applicable to purified proteins, ultimately enhancing the reliability and reproducibility of data in downstream applications involving these proteins.
Programme
We offer a 4-day course comprising lectures on protein expression and purification techniques, and methods for assessing the quality of purified protein samples. Additionally, participants will have hands-on practical sessions demonstrating how to apply these experiments for protein quality control. Topics included in the course:
Lectures
Day 1
Protein expression, purification and QC; Overview of protein expression methods; Overview of protein purification methods; Guidelines for protein quality control
Day 2
PAGE, Protein concentration and optical properties of protein; Determination of protein concentration; Polyacrylamide gel electrophoresis;
Absorbance, fluorescence, circular dichroism; Hydrogen-deuterium exchange applications in protein quality control
Day 3
Quality control tests; Light scattering and mass photometry; Mass spectrometry;
Day 4
Storage, formulation and extended tests; Differential scanning fluorimetry; Protein storage and formulation; Protein-protein interactions
Practical sessions
DLS, nanoDSF, SEC-MALS (Session 1)
Mass spectrometry, mass photometry (Session 2)
UV absorbance, fluorescence, circular dichroism (Session 3)
Application
Limited to 18 participants.
To apply, send to icpqcsiena2025@gmail.com an application package composed of 1/ a motivation letter (max. 1 page), 2/ their CV and 3/ a letter of support from your supervisor (if applicable).
Note that priority will be given to junior researchers, namely PhD students and postdocs.
Deadline for registration is April 30th, 2025
Registration
Registration fees (500 €) include admission, course materials, lunches, coffee breaks and a social dinner.
Acceptance to the course will be announced no later than May 26th, 2025. Payment should be made after acceptance.
Participants must book and pay for their own accommodation and travel expenses.
